Reporter

Part:BBa_K863113:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)

Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 978


Design Notes

This Biobrick is the result of a Standard 25 assembly of the BioBricks BBa_K863112 and BBa_K863121 and was designed to express the cellulose binding domain of Clostridium Cellulovorans binding protein (CBDclos) with a fused GFP as a reporter protein under the control of a T7-promoter BBa_K525998. 6 additional conserved bases (2 AS) were isolated upstream of the cellulose binding domain and 6 bases downstream of the binding protein gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.

Source

The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and BBa_K863121 as template for the GFP-fusion protein.

References